DNA

Part:BBa_K4630110:Design

Designed by: Zhejun Qin   Group: iGEM23_WHU-China   (2023-10-10)


stgRNA-cassette 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the first cassette, it contains Lac promoter to initiate the induced edition in the proof-of-concept. Also, the double terminator prevents it from expressing the next cassette.


Source

Complete synthesis.

References